Fig. 3
From: Olfactory sensory axons target specific protoglomeruli in the olfactory bulb of zebrafish
Mapping axon projections by sparse labeling of OMP class OSNs. a A single OSN in a 3 dpf embryo was co-labeled by transient transfection with a combination of OMP:Gal4 and UAS:Citrine plasmids and hybridization with a cocktail of or111 subfamily in situ probes (arrow head). The CZ protoglomerulus is visible as a cell free zone in the olfactory bulb (arrow). b Single OMP:Gal4;UAS:Citrine labeled OSNs project to the CZ, DZ, LG3, and MG protoglomeruli (blue bars). Those that are co-labeled with a cocktail of or111 subfamily probes project exclusively to the CZ protoglomeruls (red bars). The numbers of cells observed for each condition are noted within the bars. SEM is indicated. Scale bar: 20 μm