Fig. 5

Prdm13 expression in the retina upon Ptf1a gain and loss of function. a Double fluorescent in situ hybridization with prdm13 (red) and ptf1a (green) probes on wild type retina at stage 35/36 and 40. Right panels are enlargement of central or peripheral retina (white squares). Arrows show double labelled cells. b Quantification of the percentage of ptf1a ⁺ cells among the prdm13 ⁺ cell population (top graph) and the percentage of prdm13 ⁺ cells among the ptf1a ⁺ cell population (bottom graph) at different stages of retinogenesis. Data are presented as mean ± SEM. Number of analysed sections is indicated in each bar. c Analysis of gad1, prdm13 and glyt1 expression on stage 41 retinal transversal sections following whole mount in situ hybridization on embryos injected with ptf1a-MO, control-MO, GFP mRNA (control) or ptf1a-GR mRNA. Dexamethasone was added at stage 21/22 to activate the Ptf1a-GR fusion protein. Scale bar represents 100 μm