Fig. 2

Prdm13 is expressed in glycinergic- and GABAergic-amacrine cells. a Double in situ hybridization with prdm13 (red) and gad1 (green) probes on stage 39/40 retinal section. bIn situ hybridization with prdm13 probe (red) coupled with anti-Glycine immunostaining (green) on stage 41/42 retinal section. Arrows point to double labelled cells. Dotted lines separate the GCL and the INL. c Quantification of the percentages of GABA (gad1 ⁺), Glycine (Gly) and Calretininin (Cal) amacrine cells among prdm13 ⁺ cells in the INL and in the GCL. Data are presented as mean ± SEM. Number of analysed sections is indicated in each bar. GCL: Ganglion Cell Layer, INL: Inner Nuclear Layer. Scale bar represents 25 μm