Fig. 7

The post-injury JNK signaling pathway is turned on after proximal and distal axotomies. Class IV ddaC neurons expressing mCD8-RFP and puc-GFP were subjected to either proximal (top) or distal axotomy (bottom) as shown in the schematic diagrams. a Example images of nuclear puc-GFP signal after proximal axotomy are shown at the 0 h, 8 h and 24 h time-points. Asterisks indicate nuclei of the injured ddaC neurons. b Example images of puc-GFP signal after distal axotomy are shown at the 0 h, 8 h and 24 h time-points. c The graph shows the quantification of puc-GFP intensity in the nucleus of the injured ddaC neurons at different time-points, after normalization to the intensity of puc-GFP in the uninjured ddaC neuron in the neighboring segment of the same larva. Average intensities are shown in the bar graph, and error bars are standard deviations