Fig. 2

Sox9+ Neural Progenitors Give Rise to Neurons of all Cortical Layers. (a-e) Sox9+ neural progenitor cohorts and their progeny were labeled via tamoxifen injection of dams at E11.5, and pups were sacrificed at P0.5 (E11.5Tam; P0.5). Sox9+ progenitor-derived neurons were visible in all cortical layers and are shown with selected colocalized molecular markers in P0.5 cortex: (a) Reelin; (b) Cux1; (c) Satb2; (d) Ctip2; and (e) Tbr1. (a’-e’) Higher magnification views of boxed areas in (a-e) demonstrate colabeled cells (yellow). (f-j) Quantification of cortical immuno-labeling is shown as bin analysis for the number of cells positive for expression of each of the molecular markers in (a-e). Typically, bin 1 represents the marginal zone/ layer 1, bins 2–3 represent layers 2–4, bins 4–6 represent layer 5, bins 7–9 represent layer 6, and bin 10 represents the subplate. (k-o) Bin analysis of co-labeled cells (yellow) expressing both Ai14 (tdTomato, red) and each of the molecular markers (green)