Fig. 7

Impaired cell proliferation in the Gsx2 cKO septum. a-d Gsx2 cKO septum showed reduced cell proliferation in the SVZ, but not in the VZ, at E15.5, as revealed by Ki67 and BrdU staining after one-hour BrdU labeling. The VZ was defined according to the apical ventricular surface and the basal region where S-phase BrdU⁺ nuclei were enriched. The same size box was placed adjacent to the VZ to represent the SVZ. e-g Cell cycle retention index was relatively normal in the VZ but decreased in the SVZ of the Gsx2 cKO septum 24 h after E15.5 BrdU administration. Data represent the mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.005