Fig. 3

Progenitors and clones arise from Müller glia. a-g Micrographs of retinal sections of Tg(gfap:GFP) lines, with Müller glia cells (green) stained for PCNA (proliferating cell nuclear antigen red). As previously published, the needle stick injury causes proliferation in Müller glia (a). In our newly established genetic injury, PCNA labelled proliferation was also in Müller glia (b). c-h) A detailed time series and quantification (h) shows the peak proliferative stage during 5–7 days post-injury (dpi) (n = 12 larvae per timepoints 1–10 dpi, n = 8 larvae per timepoints 11–14, n = 6 larvae at 21 dpi). Proliferative cells in the first 5 dpi also almost exclusively co-labelled with progressively weaker GFAP:GFP, after which time there were also many proliferative cells that no longer expressed detectable GFAP:GFP. White insets (c’-g’) show higher power magnification of boxed region indicated in c-g. Results are mean ± SEM. ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer. Scale bar B (for a-b) = 50 μm, scale bar in G (for c-g) = 50 μm, scale bar G’ (for c’-g’) = 200 μm