Fig. 6.

EphrinB3 inversely controls the ratio between pMN and p3 progenitors. a, b. Transverse sections of Efnb3 ^+/- (a) and Efnb3 ^-/- (b) E11.5 embryos were immunostained to detect Olig2 (red) and Nkx2.2 (blue). c. The number of Olig2⁺, Nkx2.2⁺ and Olig2⁺/ Nkx2.2⁺ (double) progenitors was quantified. Total refers to the sum of Olig2⁺ and Nkx2.2⁺ progenitors. d. Quantification of the proportion of sections showing an overlap in Efnb3 ^+/- and Efnb3 ^-/- embryos. e. Example of spatial positioning of Olig2⁺and Nkx2.2⁺ progenitors in an overlap situation in an Efnb3 ^-/- embryo. f. Quantification of the surface of overlap between Olig2⁺ and Nkx2.2⁺ domains in Efnb3 ^+/- and Efnb3 ^-/- embryos. Error bars indicate s.e.m. (n=5 embryos per genotype); *P<0.05; **P<0.01; (Mann-Whitney-Wilcoxon test), ns: non significant. Scale bars: 50 μm.