Fig. 5.

Efnb2 interacts with Shh to control the ratio between pMN and p3 progenitors. a, b. Transverse sections of Efnb2 ^lox/GFP (a) and Efnb2 ^lox/GFP ;Olig2-Cre (b) E11.5 embryos were immunostained to detect Olig2 (red) and Nkx2.2 (blue). c. The number of Olig2⁺, Nkx2.2⁺ and Olig2⁺/ Nkx2.2⁺ (double) progenitors was quantified (n=5 embryos per genotype). Total refers to the sum of Olig2⁺ and Nkx2.2⁺ progenitors. Error bars indicate s.e.m.; **P<0.01 ns= non significant (Mann-Whitney-Wilcoxon test). d-g. Transverse sections of E11.5 embryos of different genotypes (as indicated) were immunostained for Olig2 (red) and Nkx2.2 (blue). h. Quantification of the number of Olig2⁺, Nkx2.2⁺ and Olig2⁺/ Nkx2.2⁺ (double) progenitors was quantified for each genotype (n=5 embryos per genotype). Total refers to the sum of Olig2⁺ and Nkx2.2⁺ progenitors. Error bars indicate s.e.m.; *P<0.05; **P<0.01; (Mann-Whitney-Wilcoxon test). Scale bars: 50 μm.