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Fig. 4. | Neural Development

Fig. 4.

From: Eph/Ephrin Signaling Controls Progenitor Identities In The Ventral Spinal Cord

Fig. 4.

No change in pMN proliferation or differentiation rates in absence of ephrinB2. a, b. Transverse sections of E10.5 Efnb2 lox/GFP (a) and Efnb2 lox/GFP ; Olig2-Cre (b) embryos were immunostained to detect Olig2 (red) and BrdU (green). c. Quantification of BrdU+ nuclei in the Olig2+ population in both genotypes. d, e. Transverse sections of E11.5 Efnb2 lox/GFP (d) and Efnb2 lox/GFP ; Olig2-Cre (e) embryos were immunostained to detect Olig2 (red), Islet 1/2 (blue) and BrdU (green). f. Quantification of BrdU+ nuclei in the Olig2+ population in both genotypes. g. Quantification of Islet 1/2+ nuclei in the intermediate zone relative to the Olig2+ population in both genotypes. Error bars indicate s.e.m. (n=4 embryos per group); ns= non significant (Mann-Whitney-Wilcoxon test). Scale bars: 50 μm.

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