Fig. 4.

No change in pMN proliferation or differentiation rates in absence of ephrinB2. a, b. Transverse sections of E10.5 Efnb2 ^lox/GFP (a) and Efnb2 ^lox/GFP ; Olig2-Cre (b) embryos were immunostained to detect Olig2 (red) and BrdU (green). c. Quantification of BrdU⁺ nuclei in the Olig2⁺ population in both genotypes. d, e. Transverse sections of E11.5 Efnb2 ^lox/GFP (d) and Efnb2 ^lox/GFP ; Olig2-Cre (e) embryos were immunostained to detect Olig2 (red), Islet 1/2 (blue) and BrdU (green). f. Quantification of BrdU⁺ nuclei in the Olig2⁺ population in both genotypes. g. Quantification of Islet 1/2⁺ nuclei in the intermediate zone relative to the Olig2⁺ population in both genotypes. Error bars indicate s.e.m. (n=4 embryos per group); ns= non significant (Mann-Whitney-Wilcoxon test). Scale bars: 50 μm.