Fig. 3.From: Eph/Ephrin Signaling Controls Progenitor Identities In The Ventral Spinal CordEphrinB2 controls the number of pMN progenitors and their progeny. a, b. Transverse sections of wild type (a) and Efnb2 -/- (b) E9.5 embryos were immunostained to detect Olig2. c, d. Transverse sections of Efnb2 lox/GFP (c) and Efnb2 lox/GFP ;Olig2-Cre (d) E10.5 embryos were immunostained to detect Olig2. e, f. Transverse sections of Efnb2 lox/GFP (e) and Efnb2 lox/GFP ;Olig2-Cre (f) E11.5 embryos were immunostained to detect Olig2. g. The number of Olig2+ progenitors was quantified for all genotypes. Error bars indicate s.e.m. (n=3 embryos per genotype at E9.5; n=4 embryos per genotype at E10.5; n=5 embryos per genotype at E11.5); **P<0.01, ns= non significant (Mann-Whitney-Wilcoxon test). h, i. Transverse sections of E12.5 Efnb2 lox/GFP (h) and Efnb2 lox/GFP ; Olig2-Cre (i) embryos were immunostained to detect Foxp1 (green) and Islet 1/2 (red). j. Quantification of the total number of motor neurons (Foxp1+ and Islet 1/2+) in both genotypes. k. Repartition of motor neurons in motor columns in both genotypes. Error bars indicate s.e.m. (n=6 embryos per group); *P<0.05; **P<0.01; ns= non significant (Mann-Whitney-Wilcoxon test). l-o. Transverse sections of E13.5 Efnb2 lox/GFP (l, n) and Efnb2 lox/GFP ; Olig2-Cre (m, o) embryos were immunostained to detect Olig2 (red) and NeuN (green). n, o are zoomed areas indicated by a box in m, n respectively. p. Quantification of the total number of Olig2+ cells in the mantle zone in both genotypes. Error bars indicate s.e.m. (n=4 embryos per group); **P<0.01 (Mann-Whitney-Wilcoxon test). Scale bars A-I: 50 μm; L-O: 200 μm.Back to article page