Skip to main content
Fig. 3. | Neural Development

Fig. 3.

From: Eph/Ephrin Signaling Controls Progenitor Identities In The Ventral Spinal Cord

Fig. 3.

EphrinB2 controls the number of pMN progenitors and their progeny.

a, b. Transverse sections of wild type (a) and Efnb2 -/- (b) E9.5 embryos were immunostained to detect Olig2. c, d. Transverse sections of Efnb2 lox/GFP (c) and Efnb2 lox/GFP ;Olig2-Cre (d) E10.5 embryos were immunostained to detect Olig2. e, f. Transverse sections of Efnb2 lox/GFP (e) and Efnb2 lox/GFP ;Olig2-Cre (f) E11.5 embryos were immunostained to detect Olig2. g. The number of Olig2+ progenitors was quantified for all genotypes. Error bars indicate s.e.m. (n=3 embryos per genotype at E9.5; n=4 embryos per genotype at E10.5; n=5 embryos per genotype at E11.5); **P<0.01, ns= non significant (Mann-Whitney-Wilcoxon test). h, i. Transverse sections of E12.5 Efnb2 lox/GFP (h) and Efnb2 lox/GFP ; Olig2-Cre (i) embryos were immunostained to detect Foxp1 (green) and Islet 1/2 (red). j. Quantification of the total number of motor neurons (Foxp1+ and Islet 1/2+) in both genotypes. k. Repartition of motor neurons in motor columns in both genotypes. Error bars indicate s.e.m. (n=6 embryos per group); *P<0.05; **P<0.01; ns= non significant (Mann-Whitney-Wilcoxon test). l-o. Transverse sections of E13.5 Efnb2 lox/GFP (l, n) and Efnb2 lox/GFP ; Olig2-Cre (m, o) embryos were immunostained to detect Olig2 (red) and NeuN (green). n, o are zoomed areas indicated by a box in m, n respectively. p. Quantification of the total number of Olig2+ cells in the mantle zone in both genotypes. Error bars indicate s.e.m. (n=4 embryos per group); **P<0.01 (Mann-Whitney-Wilcoxon test). Scale bars A-I: 50 μm; L-O: 200 μm.

Back to article page