Fig. 2

Developmental remodeling of the IHF does not occur in marsupials. a Immunofluorescence for Laminin (green), counterstained with DAPI (blue) shows the length of the IHF and the extent of fused septum (brackets) in horizontal sections of the forebrain from E16 and adult mice (upper panels), as well as P24 and adult dunnarts (lower panels). Insets in right-hand panels show Laminin expression (white) at adult stages within the IHF. b Schematic of midline and IHF length measurements taken to quantify normalized IHF length. c Quantification of normalized IHF length in E16 and adult mouse brains, compared with normalized IHF length in dunnarts at the equivalent stages (P24 and adult). Data are represented as means ± SEM (n-values within bars). Scale bars within insets = 100 μm