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Fig. 4 | Neural Development

Fig. 4

From: Afadin controls cell polarization and mitotic spindle orientation in developing cortical radial glia

Fig. 4

Afadin regulates mitotic spindle orientation in cortical progenitors at E13.5. a Samples of 3D-reconstruction of mitotic progenitors illustrating three different categories of mitotic spindle orientation in cell division. Cell borders are outlined with α-tubulin (cyan). ß-tubulin (red), phospho-histone H3 (green) and DAPI (blue) mark centrosomes, chromosomes and nuclei, respectively. b Bin distribution of individual spindle orientations in cortical progenitors at the ventricular surface of Mllt4 fl/fl and Emx1-Cre; Mllt4 fl/fl brains do not show significant difference at E12.5. c Distributions of mitotic spindle angles in control and mutant cells are similar at E12.5 (mean ± s.e.m. Mann–Whitney test). d Bin distribution of individual spindle orientations in cortical progenitors at E13.5 reveals that compared to controls, the mutant brain exhibits a much more random distribution of mitotic spindle division plane angles. e Distributions of mitotic spindle angles in control and mutant cells indicate that the average mitotic plane differs significantly between control and mutant at E13.5 (mean ± s.e.m. Mann–Whitney test)

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