Fig. 4
The effects of p27kip1 and Rnd2 abrogation on the morphology of Rp58 siRNA-treated embryonic cortical neurons. a–e Representative images of IZ neurons from each treatment group. f There is a significant interaction between the distribution of round, uni/bipolar and multipolar cell shapes and treatment condition (F8,60 = 20, p < 0.0001, Two-way ANOVA followed by Bonferroni’s posthoc t-test, >300 cells counted from 3 to 4 independent brains per condition), with a significant increase in the proportion of multipolar cells and a concomitant decrease in uni/bipolar shaped cells upon Rp58 siRNA treatment (p < 0.001 Bonferroni posthoc t-test). g–k Representative images of CP neurons from each treatment group. l Pie charts plotting distribution of the lengths of leading processes of uni/bipolar shaped neurons within the CP for each treatment condition. m There is a significant interaction between the distribution of round, uni/bipolar and multipolar cell shapes and treatment condition (F8,60 = 12, p < 0.0001, Two-way ANOVA followed by Bonferroni’s posthoc t-test, >300 cells counted from 3 to 4 independent brains per condition), with a significant increase in the proportion of round cells and a concomitant decrease in multipolar shaped cells upon Rp58 siRNA treatment (p < 0.001 Bonferroni posthoc t-test). n The average lengths of leading process of uni/bipolar shaped neurons is decreased upon Rp58 siRNA treatment, and this is restored by co-treatment with p27kip1 and Rnd2shRNA1 or RhoA(N19) (F4,1187 = 211, p < 0.0001, One-way ANOVA, >160 neurons analysed per condition). Scale bars represent 20 μm