Fig. 5

Expression of Sema6A, PlxnA2 and PlxnA4 in corridor cells and other subpallial structures at E13.5. a Diagram illustrating the spatial expression patterns of LGE- and MGE-derived neural population markers. The transcription factors Ebf1, Islet1, and Meis2 are detected in striatal and corridor regions of the vTel (light purple), both derivatives of the LGE, but not in the GP and the ventricular/subventricular zone of the MGE (dark purple). These territories in turn express a transcription factor, Nkx2-1, not present in LGE-derived territories. (Adapted from López-Bendito et al. [22].). b, c Double immunohistochemistry for the corridor cell marker Islet1 (red) and Sema6A (green) on coronal wild-type brain sections demonstrates the expression of Sema6A on corridor cells (Co) during the growth of TCAs into subpallial populations. Sema6A is also highly expressed in globus pallidus (GP) cells (c). d–g Double immunohistochemistry for PlxnA2 (green) and Islet1 (red) (d, e), or PlxnA4 (green) and Islet1 (red) (f, g) on coronal wild-type brain sections indicates a strong presence of PlxnA2 within the corridor and in the globus pallidus (e); PlxnA4 is also moderately present on most dorso-medial corridor domains (g), and in the lateral half of the globus pallidus area (f). Both molecules are additionally lightly expressed in the vTel subventricular zone and pial surface, in an area close to the IC, and in a discrete band at the ventral edge of the striatum (PlxnA4 is particularly present here) (d, f). Scale: 150 μm