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Fig. 4 | Neural Development

Fig. 4

From: Semaphorin-Plexin signaling influences early ventral telencephalic development and thalamocortical axon guidance

Fig. 4

Expression of Sema6A and PlxnA4 on thalamic neurons and TCAs during axonal growth into the subpallium. a–b’ Double immunohistochemistry for Sema6A (red) and neurofilament (green) on wild-type E13.5 coronal brain sections indicates expression of Sema6A in thalamic neurons, in particularly in dorso-lateral populations (b, b’), as well as on extending TCAs; the protein can also be found in vTel areas ventral to the IC (a, a’). c–d’ Double immunohistochemistry for Sema6A (red) and neurofilament (green) on coronal sections of wild-type E14.5 brains shows expression of Sema6A extending to all thalamic nuclei (d, d’); the protein is furthermore expressed along TCAs positioned more caudally along the rostro-caudal axis (d, d’), while it is not found on TCAs projecting more rostrally (c, c’). e–f’ Double immunohistochemistry for PlxnA4 (red) and neurofilament (green) in wild-type E13.5 coronal brain sections reveals that expression of PlxnA4 is mostly concentrated in medial thalamic neural populations, and is present on TCAs. Immunostaining can also be observed on some fibers in the IC contacting the axon bundle dorso-medially and ventro-laterally, and in mantle and pial surface areas of the caudal vTel (e, e’). g–h’ Double immunohistochemistry for PlxnA4 (red) and neurofilament (green) on coronal sections of wild-type E14.5 brains demonstrates localization of PlxnA4 in medial thalamic neural populations (h, h’), as well as along TCAs projecting rostrally (g, g’). PlexinA4 seems to be further localized in some caudally-located TCAs (h, h’). i–j Immunohistochemistry for PlxnA4 (red) on coronal sections of Sema6a −/− E15.5 brains shows the presence of PlxnA4 on caudally-located TCAs extending within the IC (empty arrowhead), which presumably correspond to VB-originated axons. On the other hand, no immunostaining can be detected on misrouted projections corresponding to dLGN-originated fibers (filled arrowheads). Scale: a–b’, 300 μm; c–d’, 300 μm; e–f’, 300 μm; g–h’, 300 μm; i–j, 300 μm

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