Fig. 5
From: Mutation of Kinesin-6 Kif20b causes defects in cortical neuron polarization and morphogenesis
Morphological changes in Kif20b mutant neurons are not due to cell type changes. A Representative field image of E14.5 cortical cells cultured for 2 days in vitro, immunostained for neuronal marker beta-III-tubulin (Tuj1, red), layer 5/6 marker Ctip2 (green) and nuclei (DAPI, blue). Filled arrow points to Ctip2+ neuron; open arrow to Ctip2- neuron. Scale bars, 100 μm and 10 μm in inset. B The percentage of cells in control (+/-, gray bar) and mutant (-/-, white bar) cortical cultures expressing neuronal marker (TuJ1+) is not significantly different. n = 3 coverslips each with 1120 control cells and 769 mutant cells analyzed. C The percentage of neurons marked by Ctip2 does not differ in control and mutant cultures. D and E The average percentage (±s.e.m.) of Ctip2+ or Ctip2- neurons at each stage in cultures from control heterozygous (+/-, gray bars) or Kif20b mutant (-/-, white bars) cortices. Mutant cultures have fewer polarized neurons (stage 3) and more multipolar neurons (stage 2) compared to control heterozygous cultures. For C, D, and E, n = 6 control (+/-) and 6 mutant (-/-) coverslips (2258 and 1335 neurons total, respectively). Error bars are ± s.e.m; *, p <0.05; **, p <0.01, n.s., not significant, t-test