Fig. 3
From: Mutation of Kinesin-6 Kif20b causes defects in cortical neuron polarization and morphogenesis
Kif20b mutant neurons are less polarized at 2 days or 4 days in vitro. A Representative images of dissociated E14.5 cortical neurons that are at Stage 1 (unpolarized), Stage 2 (multipolar), or Stage 3 (polarized; having one neurite at least twice as long as any other) after two days in vitro, and stained for neuron-specific beta-III tubulin (Tuj1, red), actin (phalloidin, green), and nuclei (DAPI, blue). B The average percentage (±s.e.m.) of neurons at each stage in cultures from control heterozygous (+/-, gray bars) or Kif20b mutant (-/-, white bars) cortices. Mutant cultures have fewer polarized neurons (stage 3) and more multipolar neurons (stage 2) compared to control heterozygous cultures. n = 6 control (+/-) and 6 mutant (-/-) coverslips from 3 independent experiments with 2258 total control and 1335 mutant cells scored. C After 4 days in vitro (4DIV), Kif20b mutant neurons are still less likely to be polarized. n = 4 control (+/-), n = 3 mutant (-/-) coverslips from 3 independent experiments; 827 control, 510 mutant neurons. D Representative images of dissociated cortical neurons stained for MAP2 and Tau1. Polarized neurons have a single, tau1-enriched neurite that is the axon. E The average percentage (±s.e.m.) of neurons with a single tau-enriched neurite (polarized) is decreased in Kif20b mutant cultures after 2 DIV. n = 3 control (+/-), 3 mutant (-/-) coverslips from 3 independent experiments; 615 control, 381 mutant neurons. *, p <0.05; **, p <0.001; ***, p <0.0001; n.s., not significant, t-test. Scale bars, 20 μm