Fig. 2
From: Mutation of Kinesin-6 Kif20b causes defects in cortical neuron polarization and morphogenesis
Kif20b expression and localization in cultured cortical neurons. A RT-PCR detects Kif20b weakly in neuron culture lysates (E14.5 plus 4DIV) after 30 cycles. Bands are stronger in MEFs. Bands are weaker in mutant samples due to the splice mutation [14]. B Immunocytochemistry for Kif20b does not detect signal in control neurons (+/+) above background seen in mutant (-/-) neurons. Images shown are of a polyclonal antibody to the full length protein [11], but are representative of staining appearance of five independent antibodies (see Methods). Scale bars, 20 μm. C Plasmids encoding GFP-tagged human KIF20B and mCherry were electroporated into mouse cortical neurons and imaged live. GFP puncta were distributed throughout the neurites and axon (arrows, inset). C’ and C”: Some GFP puncta moved anterogradely in the axon (arrows point to the same puncta at different time points). 10 μm scale bar and 5 μm in insets