Fig. 4

Cell cycle of Palladin ^-/- neural progenitors at E10.5 is shortened. A Expression of Cyclin E in Palladin ^-/- embryo brains is up-regulated at E10.5, as detected by western blotting. Expression of Cdk2 (a cell cycle enhancer) is up-regulated (B) while expression of Wee1 (a cell cycle suppressor) is down-regulated (C) in Palladin ^-/- embryo brain at E10.5, as assayed by qPCR. Error bars indicate SEM; *P < 0.05. D BrdU labeling of neural progenitors (pink) after indicated time of incubation. The proportion of BrdU-stained progenitors is higher in Palladin ^-/- neural progenitors at each time point (right panel) compared with wt (left panel). E Cell cycle of neural progenitors in wt and Palladin ^-/- at E10.5, Tc-Ts is 7.95 h in wt and 6.83 h in Palladin ^-/- neural progenitors (n = 5 at each time point). Error bars indicate SEM