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Fig. 4 | Neural Development

Fig. 4

From: The microtubule plus-end-tracking protein TACC3 promotes persistent axon outgrowth and mediates responses to axon guidance signals during development

Fig. 4

TACC3 affects axon guidance in vivo and ex vivo. a, b, confocal images of laterally-viewed whole-mount Xenopus spinal cord fluorescently labeled for acetylated tubulin, showing peripheral axon outgrowth in control (a) and TACC3 KD (b) embryos at 2 dpf. c, Quantitation of the embryos with motor neuron guidance defects (n = 5 embryos). d, Representative neural tube growth cone images of control and TACC3 OE, before and after addition of 400 ng/ml Slit2. e, Quantification of the percentage of the growth cone collapse events in control (n = 48) and TACC3 OE (n = 82) growth cones show significant reduction in growth cone collapse in TACC3 overexpressing growth cones. f, Cartoon model for the role of TACC3 at MT plus ends during axon outgrowth and guidance. Microtubule (blue) plus-ends decorated by TACC3 (green) promotes axon outgrowth, reduces axon retraction, dampens nocodazole induced reduction in MT dynamics parameters, rescues XMAP215 KD induced axon length reduction and opposes repellent guidance signals effect. *p < 0.05, **p < 0.01, ***p < 0.001. ns not significant. n = growth cone number. Scale bar, 50 μm and 5 μm

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Neural Development

ISSN: 1749-8104