Fig. 1

TACC3 promotes axon outgrowth velocity and prevents spontaneous axon retractions. a, Axon outgrowth velocity is significantly decreased in TACC3-depleted axons by 27% (n = 56) and in TACC3 OE, to a lesser extent, by 11% (n = 106) compared to control (GFP only) conditions (n = 58). b, Retraction rate increased 5 fold in TACC3 KD (n = 107) and decreased 0.6 fold in TACC3 OE (n = 155) in comparison to their corresponding non injected (n = 95) and GFP injected (n = 180) controls respectively. c, d, MT growth velocity (DMSO, n = 9, KHS-101, n = 9) (c) and axon outgrowth length (DMSO, n = 8, KHS-101, n = 12) (d) are significantly reduced by 28 and 26% respectively after acute depletion of TACC3 by the inhibitor KHS101. e, Schematic representation of GFP-tagged TACC3 full-length and deletion constructs, along with plus-end tracking ability (denoted by “+”) and impact on axon outgrowth length. f, Quantification of axon outgrowth length in cultured neural explants of GFP injected control (n = 997), full-length GFP-TACC3 (1-931aa) (n = 787), GFP-TACC3-ΔN (133–931) (n = 613), GFP-TACC3- ΔΔN (363–931) (n = 563) and GFP-ΔTACC domain (1-635aa) (n = 764). *p < 0.05, **p < 0.01, ***p < 0.001. ns not significant. n = axon/growth cone number