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Fig. 5 | Neural Development

Fig. 5

From: The Hunchback temporal transcription factor establishes, but is not required to maintain, early-born neuronal identity

Fig. 5

Loss of Hunchback from post-mitotic neurons only does not alter embryonic neuronal morphology. U1-U5 motor neuron morphology detected by CQ2-gal4 driving expression of UAS-myristoylated:GFP (green). Only the Hb+ U1 and U2 motor neurons project to the most dorsal muscles [22, 30]. a, b Wild type CQ2-gal4;UAS-myr:GFP stage 16 embryo. a The most dorsal projecting U1/U2 motor neurons extend past the tracheal dorsal trunk (dashed lines). b Dendritic projections form a thick posterior commissural fascicle (arrow) and a thin anterior commissural fascicle (arrowhead) in each segment; note faint processes in most intersegmental connectives. c, d CQ2-gal4/+; UAS-hb RNAi/UAS-myr:GFP stage 16 embryo. c The most dorsal projecting U1/U2 motor neurons extend past the tracheal dorsal trunk (dashed lines). No difference is seen in axon or dendrite morphology between wild type and CQ2-gal4 UAS-hb RNAi embryos. d Dendritic projections form a thick posterior commissural fascicle (arrowhead) and a thin anterior commissural fascicle (arrow) in each segment; note slightly reduced processes in most intersegmental connectives. Anterior up, dorsal (a,c) or ventral (b,d) midline at center of each panel, scale bar, 10 μm. e Quantification

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