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Fig. 6 | Neural Development

Fig. 6

From: Regulation of downstream neuronal genes by proneural transcription factors during initial neurogenesis in the vertebrate brain

Fig. 6

Overexpression of Ascl1 and Neurog2 caused upregulation of Nhlh1, Tagln3, Chga and Stmn2. a-p, minimum n = 3 for each gene) All brains have been dissected, flatmounted and in lateral view. a, a’, e, e’, i, l’, m, m’ Normal expression of Nhlh1, Tagln3, Chga and Stmn2 within neurons of the early axon scaffold in the control embryos, with GFP (red) specifically labelling cells that express the control plasmid (pCIG; CAGGS-IRES-nuclearGFP). Expression of Nhlh1 (b, b’), Tagln3 (f, f’), Chga (j; arrow) and Stmn2 (n; arrow) was upregulated in cells where rAscl1-IRES-nuclearGFP was ectopically expressed. (b, b’, f, f’) mAscl1 can be specifically labelled (red) to show co-expression with the target genes Nhlh1 and Tagln3. (c, c’ g, g’ k, o) Normal expression was also observed on the un-transfected (internal control) side of the same electroporated embryo. Ectopic expression of mNgn2-IRES-nuclearGFP also resulted in ectopic expression of Nhlh1 (d), Tagln3 (h), Chga (l) and Stmn2 (p). The un-transfected (internal control) was not displayed here. For abbreviations see Table 1

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