Fig. 3

Expression of proneural genes in the mouse brain from E8.5-E10.5. a-c E8.5 (lateral views), expression of Ascl1 (a) and Neurog1 (b) along the dorsal midline of the mesencephalon corresponding to the nmesV. Expression of Neurog2 (c) in the ventral brain, corresponding to the nMLF. d-i All brains have been dissected, flatmounted and in lateral view. d-f E9.5, expression of Ascl1 (d), Neurog1 (e) and Neurog2 (f). f Arrowhead indicates expression in the dorsal optic vesicle. g-i E10.5, expression of Ascl1 (g), Neurog1 (h) and Neurog2 (i) within the neuronal populations of the early axon scaffold tracts and motor neurons as delimited by dashed lines. Unfilled arrowhead indicated caudal thalamus. There were other areas of the brain that expressed Ascl1, including the ventral cortex, pretectum and prethalamus. Neurog1 and Neurog2 were both expressed in the dorsal cortex, the dorsal optic vesicle (arrowhead) and the caudal thalamus (unfilled arrowhead). j Schematic of neuronal populations and complementary expression in these early neuronal populations of Ascl1 (dark green) and neurogenins (light green) and in other regions Ascl1 (dark blue) and Neurogenins (light blue). For abbreviations see Table 1