Fig. 2

Loss of Notch signalling affects expression of Hes5, Ascl1, Nhlh1 and Tagln3 in the mouse brain. (a-d) All brains have been dissected and flatmounted in lateral view. e-h Whole mount embryos. a, b, n = 10 Expression of Hes5 at E9.5 within the embryonic mouse brain of the control (a) and RBPJ mutant (b). c, c’, d, d’, n = 10 Ascl1 expression in the neuronal populations, which give rise to the early axon scaffold tracts at E9.5 of the control (c, c’) and RBPj mutant brains (d, d’). Boxes in c and d indicate higher magnification in c’ and d’ respectively. Arrowhead indicates normal salt-and-pepper like expression of Ascl1. Control and mutant embryos were compared from the same littermates. e, f, n = 5 Nhlh1 expression in control (e) and RBPj mutant (f). g, h, n = 5 Tagln3 expression in control (g) and RBPj mutant (h). Expression of Nhlh1 and Tagln3 was upregulated throughout the brain. For abbreviations see Table 1