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Fig. 6 | Neural Development

Fig. 6

From: Akirin2 is essential for the formation of the cerebral cortex

Fig. 6

Disrupted production of cortical neurons in Akirin2 knockout embryos. a, b The number of Pax6+ progenitors in the Akirin2 knockout is comparable to controls at E10.5, but significantly reduced by E11 (a, b). c, d In contrast, there are already significantly fewer Tuj1+ neurons in knockouts at E10.5; this difference is maintained at E11. Unlike in controls, where all neurons migrate superficially to form a tight preplate (PP) band, at E11 some knockout neurons that double-stain for Tuj1 and TBR2 appear “trapped” at the apical ventricular surface (arrowheads, and inset, from c). e Neurons that do successfully migrate to the PP in the Akirin2 knockout form a less tightly-defined layer (black lines) compared with control (blue lines), indicated by a line-scan of Tuj1 intensity across the thickness of the cortical wall (e). Subsequently, Tuj1 fluorescence intensity is spread more broadly at the PP and is also more apparent at the apical ventricular surface (VS) and throughout the ventricular zone (VZ) in the Akirin2 knockout. **p < 0.01, ****p < 0.0001. LV, lateral ventricle. Scale bar: 100 μm in (a, c); 50 μm in (c) inset

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