Fig. 1

The Akirin2 gene is expressed throughout cortical development. a RT-PCR using two different primer-sets that generate amplicons across multiple exons. Akirin2 is expressed in telencephalic/cortical tissue at E11, E12, E15, P0, P10, P28 and in the adult mouse. The -RT control confirms that none of the bands are due to genomic DNA contamination. b, c Low magnification (b) and higher magnification (c) images of in situ hybridization experiments utilizing an antisense riboprobe for Akirin2 indicate that it is expressed throughout the developing telencephalon, with high expression in the early ventricular zone (vz) and in post-mitotic neurons situated in the preplate (pp; E12), intermediate zone (iz) or cortical plate (cp). No appreciable signal was observed using an Akirin2 sense probe in these or any other experiments. ctx, cortex; str, striatum. Scale bar: 1 mm in (b), 200 μm in (c)