Fig. 5
From: Oligodendrocyte development in the embryonic tuberal hypothalamus and the influence of Ascl1
Olig2+ oligodendrocyte progenitor cell populations in the developing embryonic tuberal hypothalamus at E13.5, E15.5 and E17.5. a Representative images immunolabled with antibodies to PdgfRα and Olig2 in the tuberal hypothalamic area of wildtype CD1 embryonic brains, with boxed areas magnified for the ventricular zone (VZ; area highlighted with bracket) and mantle zone (MZ). Red arrows indicate examples of Olig2+/PdgfRα- cells, green arrows indicate examples of Olig2+/PdgfRα + cells. Third ventricle outlined with dotted line. b Cell counts of Olig2+ and PdgfRα + (OPCs) or PdgfRα- (glioblasts) populations in the MZ. c Proportion of PdgfRα + co-labeled and PdgfRα- cell populations within the total Olig2+ population in the MZ. Bar graphs represent mean ± SD (n = 4–5 embryos per group; 3 brain sections per embryo). Statistics; *P < 0.01, **P < 0.001, ***P < 0.0001, ****P < 0.0001. ANOVA with Tukey Post-Hoc. Scale bars equal 100 μm