Fig. 4
From: Oligodendrocyte development in the embryonic tuberal hypothalamus and the influence of Ascl1
Glial progenitor cell populations in the developing embryonic tuberal hypothalamus at E13.5, E15.5 and E17.5. a Representative images of glioblast cells immunolabled with antibodies to Sox9 and Olig2 in the tuberal hypothalamic area of wildtype CD1 embryonic brains with boxed areas magnified for the ventricular zone (VZ; lateral area highlighted with bracket) and mantle zone (MZ). Insets show co-labeling of cells. 3rd ventricle outlined with dotted line. Glioblast cell counts of glioblast subpopulations that are (b) lining the ventricle in the VZ and c in the MZ. Proportion of glioblast subpopulations within the total glioblast population (d) lining the ventricle in the VZ and e in the MZ. Bar graphs represent mean ± SD (n = 4–5 embryos per group; 3 brain sections per embryo). Statistics; *P < 0.01, **P < 0.001, ***P < 0.0001, ****P < 0.00001. ANOVA with Tukey Post-Hoc. Scale bars equal 100 μm