Fig. 2

Olig2+ cells appear in a domain region along the 3rd ventricle in the tuberal hypothalamus. a Olig2+ cells located at the third ventricle (3 V) congregate in a domain in the tuberal area of the hypothalamus at the dorsal edge near the hypothalamic sulcus (dotted white line) at E13.5. Insets depict the Olig2 staining with the domain area indicated with arrows. b Illustration of coronal plane of nuclei of the tuberal hypothalamus to depict the hypothalamic sulcus dividing the thalamus (Th) from the hypothalamus (Hy). c Olig2+ cells at E15.5 and E17.5 with the Olig2+ domain area highlighted in the magnified image. White dotted line outlines the third ventricle. d BrdU and Olig2 co-immunostaining at E13.5 with areas dorsal to domain, the domain, and ventral to domain highlighted to outline differences in proliferative ability. e Ki67 and Olig2 co-immunostaining at E13.5 and E15.5. White arrow highlights pseudostratified proliferating cells ventral to domain, green arrow highlights a Ki67+ cell in domain area, yellow arrows indicate Ki67+/Olig2+ cells at the VZ and MZ. Scale bars equal 100 μm