Fig. 6

Dorsal interneuron migration is delayed in Ntn1, Dcc, and Robo3 knockouts. a Immunohistochemistry of BARHL2 at different stages. At E11.75, BARHL2+ neurons fail to reach the lateral spinal cord in the KOs (indicated by arrow). Their ventral migration is also delayed. At E12.5, dI1i (ipsilateral) and dI1c (contralateral) populations appear normal in WT and Robo3 KOs. There are still some neurons in the dorsal spinal cord in Ntn1 and Dcc KOs (arrow). At E13.0, all mutants have two distinct populations of dI1 neurons. b Quantification of phenotypes in (a). For quantifying the ventral migration, the distance from the dorsal margin to the ventral most BARHL2+ neurons (h1) is compared to the total height of the spinal cord (h2). For the lateral migration, the distance between the medial most and lateral most BARHL2+ neurons (w1) is compared to the distance between the medial most neurons and the lateral margin of the spinal cord (w2). The distances were measured using ImageJ. Data are normalized to WT and are represented as the mean ± SEM (Student’s t-test; **, p < 0.001; ns, not significant). Scale bars, 50 μm