Fig. 1

Neuroprogenitor migration in Ntn1, Dcc, and Robo3 knockouts. a Schematic of the migration of the dorsal spinal cord progenitors and interneurons. VZ, ventricular zone. b Cross sections of the spinal cord electroporated with Actb-gfp (βactin-gfp). The closeup images are of the boxed area. The embryos were cultured for 20 h. GFP+ neurons from all three KOs migrate out of the VZ (demarcated by PAX3/7 staining) later than WT neurons. c Quantification of the ratio between GFP+ neurons within the VZ and the total GFP+ neurons. A higher percentage of neurons is seen within the VZ in all three KOs. Data are represented as the mean ± SEM (Student’s t-test; **, p < 0.001; *, p < 0.05). Scale bars, 50 μm