Fig. 9
From: Contralateral migration of oculomotor neurons is regulated by Slit/Robo signaling
Robo3 or CXCR4 function is not required for oculomotor migration into the floor plate. A, B. To determine if Robo3 or CXCR4 are in position to repress Slit/Robo signaling in situ hybridization of RNA was performed on E14.5 midbrain sections. Robo3 was not expressed in the ventral midbrain on E14.5 (A). However, CXCR4 was expressed in the ventral midbrain in both the floor plate and regions lateral to the floor plate (B). C, D To identify migration patterns of oculomotor neurons on E13.5 in Robo3 and CXCR4 mutants, sections of the posterior midbrain were antibody labeled with Islet1/2. Islet1/2+ cells migrated into the floor plate in both Robo3 (C) and CXCR4 mutants (D) indicating neither Robo3 nor CXCR4 are required for migration oculomotor migration across the floor plate. (Robo3, n = 2; CXCR4, n = 4) Scale bar, 100 μm