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Fig. 7 | Neural Development

Fig. 7

From: Contralateral migration of oculomotor neurons is regulated by Slit/Robo signaling

Fig. 7

Loss of slit 2 in motor neurons is not sufficient to cause premature migration. A, B To confirm a loss of Slit2 expression in the oculomotor nucleus in Islet1 mutant embryos, in situ hybridization was performed in the ventral midbrain on E11.5. C, D. Location of the oculomotor nucleus was determined by Phox2b expression (an Islet-independent transcription factor expressed in motor neurons). A In control embryos, Slit2 RNA is found in the floor plate and co-localized to Phox2b positive cells (C). B In the Islet1 mutant midbrain, Slit2 expression is retained in the floor plate, but is lost from the oculomotor nucleus (D). Phox2b positive neurons were clustered on either side of the floor plate, but not within the floor plate in both control and Islet1F/F mutants (B) indicating oculomotor cell bodies have not migrated into the floor plate (n = 4). Scale bar, 100 μm

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