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Fig. 11 | Neural Development

Fig. 11

From: Contralateral migration of oculomotor neurons is regulated by Slit/Robo signaling

Fig. 11

Oculomotor midline migration is independent of peripheral signals. A. Open book preparation of the midbrain in a mouse lacking extraocular muscles in Pitx2 mutant mice, with the oculomotor nucleus back labeled with DiI. On E14.5, a commissure originating from the posterior half of the oculomotor nucleus crossed the floor plate (n = 3). B, C. Explant culture of isolated midbrain tissue. E10.5 midbrains were dissected to remove all peripheral tissue, including nIII, then cultured in an open book preparation in collagen gel for 72 h. Cultured tissue was then labeled with Islet1/2 antibody. Anterior is up; floor plate indicated by bracket. B. Dissected tissue at the onset of culture period showed Islet1/2+ cell bodies on either side of the floor plate. C. Following incubation for 72 h, a posterior subset of oculomotor neurons migrated into the midline (n = 4). Brackets indicate floor plate region. Scale bars, 100 μm

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