Fig. 1

The axons of newly produced gcs stack inside to out in the molecular layer. a-b Adjacent sagittal midline sections of a P28 Atoh1-tau Cb after Tm administration at P10 stained for (a) X-gal to visualize the nuclei of the marked cells, (b) GFP fluorescent immunostaining (green) to visualize the axons of the marked granule cells. The anteroposterior zones have been color coded (anterior zone: green, central zone: blue, posterior zone: purple and nodular zone: yellow). c-h sagittal midline sections of lobule 3 of P28 Atoh1-tau Cb stained for GFP to reveal the position of the axons of the marked granule cells after Tm administration at the indicated ages showing that the proportion of molecular layer labeled diminishes as Tm is administered later