Fig. 3
From: In vivo functional analysis of Drosophila Robo1 immunoglobulin-like domains
Robo1 Ig domains are not required for regulation by Comm. a–h Stage 16 embryos stained with anti-HRP (magenta) and anti-HA (green) antibodies. Lower images show HA channel alone from the same embryos. Embryos carrying one copy of the indicated robo1 transgenes along with elav-GAL4 display normal expression of the HA-tagged Robo1 variants (a-d, arrows). Embryos carrying one copy of the indicated robo1 transgenes along with elav-GAL4 and UAS-Comm display strong reduction in axonal HA expression and a slit-like midline collapse phenotype reflecting increased midline crossing (e-h, arrows with asterisk). Pairs of sibling embryos shown here (a and e; b and f; c and g; d and h) were stained in the same tube and imaged using identical confocal settings to allow an accurate comparison of HA levels between embryos