Fig. 2
From: In vivo functional analysis of Drosophila Robo1 immunoglobulin-like domains
Robo1 Ig2-5 domains are not required for axonal localization and exclusion from commissures in wild-type embryos. a Schematic of the robo1 rescue construct (Brown et al., 2015). HA-tagged Robo1 variants are expressed under the control of regulatory regions from the robo1 gene. All transgenes are inserted into the same genomic landing site at cytological position 28E7. b–g Stage 16 embryos stained with anti-HRP (magenta) and anti-HA (green) antibodies. Bottom images show HA channel alone from the same embryos. HA-tagged full-length Robo1 (b) and each of the Ig domain deletion variants (c–g) expressed from the robo1 rescue transgene in a wild-type background are localized to longitudinal axon pathways (arrowhead) and excluded from commissural axon segments in both the anterior commissure (AC, white arrow) and posterior commissure (PC, black arrow). Robo1∆Ig3 expression is elevated within neuronal cell bodies compared to the other transgenes (e, arrowhead with asterisk)