Lmx1b is required for the glutamatergic fates of a subset of spinal cord neurons

Table 5 lmx1ba and lmx1bb mutant alleles act redundantly in a dose-dependent manner

	48 h	lmx1ba ^+/+ lmx1bb ^+/+	lmx1ba ^+/- lmx1bb ^+/+	lmx1ba ^+/+ lmx1bb ^+/-	lmx1ba ^+/- lmx1bb ^+/-	lmx1ba ^-/- lmx1bb ^+/+	lmx1ba ^+/+ lmx1bb ^-/-	lmx1ba ^-/- lmx1bb ^-/-
slc17a6	Mean	246.3	250.2	233.8	196.4	199.4	204.5	193.1
	SEM	7.4	5.9	4.5	5.5	10.4	10	6.2
	n	8	5	4	8	11	9	13
	p value 1	n/a	0.72	0.3	0.001	0.001	0.003	0.001
	p value 2	0.3	0.08	n/a	0.005	0.03	0.05	0.004
	p value 3	0.001	0.001	0.005	n/a	0.66	0.38	0.78
	p value 4	0.003	0.004	0.05	0.38	0.7	n/a	0.45
	p value 5	0.001	0.001	0.03	0.66	n/a	0.7	0.78
slc32a1	Mean	179.8						188.5
	SEM	12.9						14.1
	n	6						6
	p value 1	n/a						0.94

Mean number of slc17a6a + slc17a6b (slc17a6) or slc32a1-expressing cells detected in the spinal cord region adjacent to somites 6–10 in 48 h embryos. SEM is the standard error of the mean. n is the number of embryos analyzed for each data set. p values are from student’s paired t-test. Statistically significant p values are indicated in bold. p value 1 is from comparing WT (lmx1ba ^+/+ ;lmx1bb ^+/+) embryos pairwise with all other genotypes, p value 2 is from comparing lmx1ba ^+/+ ;lmx1bb ^+/- embryos pairwise with all other genotypes, p value 3 is from comparing lmx1ba ^+/- ;lmx1bb ^+/- embryos pairwise with all other genotypes, p value 4 is from comparing lmx1ba ^+/+ ;lmx1bb ^-/- embryos pairwise with all other genotypes and p value 5 is from comparing lmx1ba ^-/- ; lmx1bb ^+/+ embryos pairwise with all other genotypes

ISSN: 1749-8104