Fig. 6
From: Lmx1b is required for the glutamatergic fates of a subset of spinal cord neurons
lmx1bb is expressed by dI5 and V0v neurons and these cells form in normal numbers in lmx1bb mutants. Lateral view of zebrafish spinal cord at 48 h (a-f), anterior left, dorsal top. in situ hybridization for lbx1a in WT (a) and lmx1bb homozygous mutant (b) embryos. in situ hybridization for evx1 + evx2 (evx) in WT (c) and lmx1bb homozygous mutant (d) embryos. Immunohistochemistry for EGFP in Tg(evx1:EGFP) SU1 WT (e) and lmx1bb homozygous mutant (f) embryos. g Mean number of cells (y-axis) in WT embryos (white) and lmx1bb homozygous mutants (grey) expressing lbx1a, evx or Tg(evx1:EGFP) SU1 in spinal cord region adjacent to somites 6–10 (x-axis). Two independent experiments were conducted for (a-f). Cell count results were similar in each replicate. Data shown here (g) are average values of 6–10 embryos from the same experiment. Precise number of embryos counted and p values are provided in Table 4. Error bars indicate standard error of the mean. Scale bar = 50 μm (a-f)