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Fig. 1 | Neural Development

Fig. 1

From: Dual leucine zipper kinase regulates expression of axon guidance genes in mouse neuronal cells

Fig. 1

Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. a The relative mRNA level of DLK in infected cells was analyzed by quantitative RT-PCR, normalized to three housekeeping genes and calculated with the ΔΔC T method. The value of DLK mRNA expression in control cells (pLKO.1) was arbitrarily set to 1. Data are the mean ± SEM (error bars) from three independent experiments carried out in triplicate. ****, p < 0.0001. b Representative Western blots showing levels of DLK, phospho-JNK (p-JNK), total JNK, phospho-c-Jun (p-c-Jun) and actin in infected Neuro-2a cells. c Quantitative densitometric measurements of DLK, p-JNK, total JNK and p-c-Jun protein levels in infected cells. Results are normalized to either actin or total JNK level in control cells, which were set to 1, and represent mean ± SEM (error bars) from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001

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