Fig. 6

Temporal-dependent fate of Dbx1-derived cells in the LH. a-d Images of the hypothalamus with YFP-expressing cells in medial and lateral portions of the LH after tamoxifen gavage at E7.5 (a), E9.5 (b), and E11.5 (c). e-l Proportionately more Pmch (e-h) and Hcrt (i-l) co-labeled with YFP when tamoxifen was given during later stages of development (E9.5-E11.5). Mean ± SEM; E7.5 n = 4 mice (2 males, 2 females); E9.5 n = 3 mice (0 males, 3 females); and E11.5 n = 9 mice (2 males, 7 females). The scale bar represents 250 μm for panels (a-c), 50 μm for low mag and 10 μm images in panels (e-k). Abbreviations: mt, mammillothalamic tract; f, fimbria; VMH, ventromedial hypothalamic nucleus; mLH, medial portion of the lateral hypothalamus; lLH, lateral portion of the lateral hypothalamus; DMH, dorsomedial hypothalamic nucleus