Fig. 3

Postnatal fate of Dbx1-derived cells in the LH in males and females. Schematic of a coronal view of the postnatal brain at the level of the LH (a) show regions analyzed in (B-E.iv). Low magnification merged panels (b, c, d, and e) are shown on the left, with high magnification of boxed area shown in right panels (i - iii). In the LH, a high percentage of Dbx1-derived neurons (green) express markers (red) Pmch (B-B.iv), Nesfatin (Nfn; C-C.iv), Cart (D-D.iv) or Hcrt (E-E.iv). The Dbx1-lineage contribution to the Cart (D.iv) and Hcrt (E.iv) populations is sexually dimorphic. Mean ± SEM; n = 3–6 mice per sex; *, p < 0.05. The scale bar represents 50 μm for low mag panels (b, c, d, and e) and 10 μm for high mag panels (i-iii)