Fig. 6
Early, but not late, mir-135a-2OE resembles mutants with Wnt deficits. a-d Coronal sections of E12.5 control (mir-135a-2OE) and mutant (Emx1::Cre;mir-135a-2OE) brains. Wnt3a (a, b) and Wnt8b (c, d) mark the cortical hem (Wnt3a + and Wnt8b+, brackets and arrows) and the hippocampus (hp) primordium (Wnt8b+, brackets). Dashed lines highlight the neocortex length from the PSB to the hem. g-h Lmx1a expression in E12.5 control (mir-135a-2OE) and Nes::Cre;mir-135a-2OE mutant brains. Dashed lines highlight the cortical hem area and the neocortical domain size. e-f and i-j Quantifications of the cortical hem area (μm2 ± SEM) along the rostro-caudal axis (n = 3) and the neocortex length (μm ± SEM) from the PSB to the hem at the same levels (n = 3) in Emx1::Cre;mir-135a-2OE (e and f) and Nes::Cre;mir-135a-2OE (i and j) mutants. ***, p <0.001; *, p < 0.05. ch, cortical hem; PSB, pallium-subpallium boundary; ncx, neocortex. Scale bar 100 μm in panels a, b and g, h; 50 μm in panels c and d