Fig. 4

Primary cilia dynamics in relation to RGC differentiation events. Schematic representation and summary of the observations made in the time-lapse experiments. A single cell is represented in each row. For each cell, the presence of its primary cilium (green) is plotted in relation to the different events during RGC differentiation: apical process retraction (cyan), axon outgrowth (dark grey) and dendrite formation (magenta). The cells that show similar behaviors are indicated in the different columns: cells that show a primary cilium before apical detachment (●: cilium disappears previous to detachment; ○: cilium remains visible and moves basally previous to detachment); cells that show a primary cilium during the retraction of the apical process (■); cells that continuously show a primary cilium through the period from apical retraction to pre-dendritic filopodial activity/dendritogenesis (▲), and those in which the cilium disappears at least for a short time during this period (∆). The dashed regions in the diagrams indicate the time interval in which the primary cilium was localized to non-apical positions. Cells that showed this behavior are indicated as “NA”, and those in which the primary cilium remained apical after the retraction of the apical process are indicated as “A”. A total of 24 cells from 14 embryos in 12 different experiments were analyzed. For a high resolution image of Fig. 4 please see Additional file 15