Fig. 6

Selective sympathetic innervation deficits in mice possessing the Gdf5 ^bp mutation. a-c Relative innervation density assessed by quantification of tyrosine hydroxylase-positive sympathetic fibres at P10 in the irides (a), submandibular glands (b) and trachea (c) of wild type mice (WT) and mice that are heterozygous (Het) or homozygous (Gdf5 ^bp) for the Gdf5 ^bp null mutation. The level of tyrosine hydroxylase staining is normalised to 100 for the tissue of wild type mice (mean ± SEM, *P < 0.01, **P < 0.00001, ***P < 0.0000001, statistical comparison with wild type, one-way ANOVA with Tukey HSD post hoc, n = 10 animals per genotype for the iris, n = 5 animal per genotype for the submandibular gland and the trachea). d Representative sections of the irides of P10 wild type and Gdf5 ^bp mice stained for tyrosine hydroxylase. The boundaries of the iris are indicated by the dashed lines. e Representative sections of the iris of a P10 wild type and Gdf5 ^bp mice double stained for GDF5 and tyrosine hydroxylase. Scale bar = 100 μm. f Representative whole mounts P10 wild type and Gdf5 ^bp mice stained for tyrosine hydroxylase. Scale bar = 100 μm. g Numbers of neurons in the SCG of P5 and P10 wild type and Gdf5 ^bp mice (n = 3 per genotype)