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Fig. 5 | Neural Development

Fig. 5

From: Charcot-Marie-Tooth 2b associated Rab7 mutations cause axon growth and guidance defects during vertebrate sensory neuron development

Fig. 5

Central axon guidance defects in transgenics expressing CMT2b Rab7 mutants. a Lateral (top two panels) and 3D rotation dorsal (bottom two panels) views of TagRFP-caax labeled RB neuron in Tg(−3.1ngn:gfp-Rab7) embryo at 23 hpf showing ascending and descending ipsilateral projections of central axons. Two bilateral rows of RB cells (green) are best seen in dorsal views. b-c Lateral (top two panels) and 3D rotation dorsal (bottom two panels) views of TagRFP-caax labeled RB neurons in Tg(−3.1ngn:gfp-Rab7 L129F) embryos at 23 hpf. b Misguided descending central axon (yellow arrow) turns around to travel anteriorly and dorsally from its normal pathway. c Misguided central axon (yellow arrowhead) crosses dorsal midline to join the contralateral central fascicle. All views anterior to the left. Scale bar = 40 μm. d Quantification of number of neurons with central axon guidance errors. Cont Rab7 = Tg(−3.1ngn:gfp-Rab7): 10 neurons in 7 embryos; Tg(−3.1ngn:gfp-Rab7 L129F): 43 cells in 43 embryos, *p = 0.05; Tg(−3.1ngn:gfp-Rab7K157M): 48 cells in 27 embryos, p = 0.2; Chi Square test

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Neural Development

ISSN: 1749-8104