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Fig. 5 | Neural Development

Fig. 5

From: Pax6 is required intrinsically by thalamic progenitors for the normal molecular patterning of thalamic neurons but not the growth and guidance of their axons

Fig. 5

Abnormal upregulation of Nkx2.2 by Pax6 −/− neurons in chimeras. a Diagram showing the locations of the panels in the rest of the Figure. b,c Nkx2.2 protein expression in (b) WT and (c) Pax6 −/− embryos at E16.5. Expression is normally confined to a few cells along the border of thalamus (Th) and prethalamus (PTh) and the vLG (b) but is expressed widely throughout the Pax6 −/− thalamus (c). d–g Nkx2.2 is expressed by Pax6 −/− GFP+ cells, but not by WT cells, throughout the thalamus in E16.5 chimeras. h, i Prox1 protein expression in (h) Pax6 +/+ and (i) Pax6 −/− embryos at E16.5. Prox1 is expressed in the WT thalamus and in the mutant thalamus, which is reduced in size. j, k Pax6 −/− GFP+ cells in the thalamus (e.g. arrow in j) do not show abnormal expression of Prox1. l Many of the Pax6 −/− neurons that express (abnormally) Nkx2.2 in the thalamus of chimeras co-express Prox1. m, n, o Sox2 and Lhx1/5 protein expression in (m) WT and (n) Pax6 −/− embryos at E16.5. In both genotypes, Sox2 is confined to the thalamus, which does not co-express Lhx1/5. p, q Pax6 −/− GFP+ cells in the thalamus (e.g. arrow) do not show abnormal expression of Lhx1/5, which is expressed as normal along the thalamic-prethalamic border and in the vLG. r–t Pax6 −/− GFP+ cells show normal expression of Lhx1/5 in the vLG and of Sox2 in the thalamus (r); Pax6 −/− GFP+ cells close to the border express either one or other of these markers but not both (s); Pax6 −/− GFP+ thalamic cells express Sox2, as do their WT neighbours (t). Scale bars: b-k, m, n, p, q, 50 μm; L, 5 μm; O, 15 μm; R, 25 μm; S,T, 10 μm

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