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Fig. 2 | Neural Development

Fig. 2

From: EphrinB2 sharpens lateral motor column division in the developing spinal cord

Fig. 2

cKO embryos exhibit axon guidance defects. a/ Transverse vibratome sections of E12.5 control (EfnB2 lox/GFP) (a-c) and Efnb2 cKO (EfnB2 lox/GFP; Olig2-Cre) embryos (d-f) injected with HRP into dorsal forelimb shank muscles and stained for HRP (b, e) and Islet 1 (a, d). Dotted lines indicate LMCm MN. Proportion of HRP+/Islet 1- neurons (g) in control (n = 3) and Efnb2 cKO (n = 3) embryos. b/ Transverse vibratome sections of E12.5 control (EfnB2 lox/GFP) (a-c) and Efnb2 cKO (EfnB2 lox/GFP; Olig2-Cre) embryos (d-f) injected with HRP into ventral forelimb shank muscles and stained for HRP (b, e) and Islet 1 (a, d). Dotted lines indicate LMCm MN. Proportions of HRP+/Islet 1- neurons (g) in control (n = 3) and Efnb2 cKO embryos (n = 3). Error bars indicate s.e.m.; ***P < 0.001; ns = non significant (unpaired two-sample t-tests). Scale bars: 50 μm

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